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    Mondello L. Lewis A.C., Bartle K.D. (2002) Multidimensional Chromatography.

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    Раздел: Химия >>Методы
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    Separation Science is a mature and unified subject in which now conventional chromatographic and electrically driven processes are applied in the analysis of mixtures of compounds ranging from permanent gases to proteins. The boundaries between previously distinct techniques are increasingly blurred and it is becoming very evident that is a single theory may be applicable to chromatography whatever the physical state of the mobile phase. Gas, liquid and supercritical fluid chromatography can be regarded as special cases of the same procedure, while capillary electrochromatography combines liquid chromatography with electrophoresis.

    Separation science is now very focused on reducing not only timescales for analyzis, but also the size and physical nature of the analytical device, Miniaturisation of entire analytical procedures provides a strong driving force for these trends in unifying theory and practice, and is a process likely to continue, as separations using microfluidic devices are developed. In spite of these many advances however, the complexity of many naturally occurring mixtures exceeds the capacity of any single method, even when optimized to resolve them. For many years therefore, intense effort has been concentrated on coupling separations methods together to increase resolution, and these have proceeded parallel with advances in coupling separation methods with spectroscopy. As our ability to isolate components in mixtures has increased, so has our appreciation for the shear complexity of compounds found in nature, Even separation systems with the capacity to isolate many thousands of species, are found to be inadequate when applied to commonplace mixtures such as diesel fuel. We clearly have some way to go in realising separation systems that can provide truly universal and complete separations.

    Recent advances in multidimensional separation methods have been rapid and we considered that the time was appropriate to bring together accounts by leading researchers who are developing and applying multidimensional techniques. These authors have emphasized underlying theory along with instrumentation and practicalities, and have illustrated techniques with real-world examples. We hope that the eader will be as excited as we are by this combined account of progress. We thank all our contributors for their significant efforts in producing chapters of high scientific quality. We are especially indebted to Katya Vines of John Wiley who guided the project through its early stages and more recently to Emma Dowdle who brought it to completion.

    Mondello L. Lewis A.C., Bartle K.D. (2002) Multidimensional Chromatography.

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